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Metode skrining fitokimia pdf files
Metode skrining fitokimia pdf files







DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic. This implies that antioxidative compounds other than phenolics and flavonoids were probably responsible for inhibition of DPPH. But gimbiwo and guareye extracts appeared to have low TPC, their antioxidant activities were ranked moderate to high. The highest antioxidant activity of astara extract was confirmed by the highest total phenolic content. Radical scavenging activities in terms of IC50 using DPPH radical reagent of the extracts ranged from 0.259 mg/ml of astara to 0.644 mg/ml of kibinar. Astara contained the highest TPC whereas, agade contained the highest TFC compared to others. Total phenolic content and antioxidant of local varieties (locally named Agade, Astara, Gimbwo, Guarye and Kibinar) displayed differences among them. Phytochemical screening showed similarity in chemical contents which indicated the presence of flavonoids, tannins and Cardiac glycosides. Phytochemical screening, total phenolic content and antioxidant activities of local varieties of Ensete ventricosum corm were studied.









Metode skrining fitokimia pdf files